goat anti mouse apoe polyclonal antibody m 20  (Santa Cruz Biotechnology)

Journal: Experimental eye research

Article Title: Altered expression of genes functioning in lipid homeostasis is associated with lipid deposition in NOD mouse lacrimal gland.

doi: 10.1016/j.exer.2009.03.020

Figure Lengend Snippet: Fig. 2. Differential localization of Apo-F protein in acinar cells from LGs of NOD and BALB/c mice. Cryosections of LGs from 12- or 4-week-old male NOD and matched BALB/c mice were labeled with rabbit anti-mouse Apo-F polyclonal antibody and rat anti-Lamp2 monoclonal antibody followed by fluorophore-conjugated secondary antibodies. Nuclei were stained with DAPI (blue) and actin filaments with fluorescent phalloidin (purplish) in all panels to delineate cellular organization within the tissue. The sections were imaged by confocal fluorescence microscopy. Labels on the panels indicate the mouse strain and age. Arrowheads point to the Lamp2-positive (green) late endosomes/lysosomes; arrows to Apo-F-positive (red) bead-like structures near to the basolateral membrane of the acinar cells from 12-week BALB/c mice, to faint perinuclear reticular staining in 4-week BALB/c mice and to Apo-F-positive labeling in secretory vesicle-like organelles within the acinar cells from 12- and 4-week NOD mice. Bar, 10 mm. The controls labeled with secondary antibodies showed no fluorescence for Apo-F (data not shown).

Article Snippet: Goat anti-mouse Apo-E polyclonal antibody (sc-6384) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); rat anti-Lamp2 monoclonal antibody (ab13524) purchased from Abcam (Cambridge, MA).

Techniques: Labeling, Staining, Microscopy, Membrane